An analysis of contractile proteins in developing chick heart by SDS polyacrylamide gel electrophoresis and electron microscopy

An analysis of contractile proteins in developing chick heart by SDS polyacrylamide gel electrophoresis and electron microscopy.

Chick heart development was studied using transmission electron microscopy and SDS-polyacrylamide gel electrophoresis in combination with densitometry. Myosin heavy chain, alpha-actinin, actin and tropomyosin accumulations were analysed in developing hearts from preheartbeat stage 9 (Hamburger-Hamilton staging series) through 2 days after hatching. At the preheartbeat stage, electron microscopy...

SDS Polyacrylamide Gel Electrophoresis of Proteins.

Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes. The amount of SDS bound by a protein, and so the charge on the complex, is roughly proportional to its size. Common...

Intracellular fibres in cultured cells: analysis by scanning and transmission electron microscopy and by SDS-polyacrylamide gel electrophoresis.

The 3-dimensional structure of the fibrous cytoskeleton of 3T3 cells was examined by scanning electron microscopy of cells extracted with the non-ionic detergent Triton X-100. Detergent-extracted cells consist of the nucleus and an extensive system of fibres, the largest of which correspond to stress fibres visible by phase-contrast microscopy. The system of fibres, which is coterminous with th...

Unpredictable behaviour of mucins in SDS/polyacrylamide-gel electrophoresis.

Proteins – structure, properties and their separation by SDS- polyacrylamide gel electrophoresis

This paper deals with three basic aspects: 1) basics of protein structure in relation to physicochemical characterization, protein stability, importance of disulfide bond and their structure; 2) basic principles involved in polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE and the rationale behind the use of disc electrophoresis; and 3) detailed protocol involved in SDS-PAGE in terms of re...